产地：MBI
货号：ER0682 Conditions for 100% Activity
1X Buffer Tango™:
33 mM Tris-acetate (pH 7.9 at 37°C), 10 mM Mg-acetate, 66 mM K-acetate and 0.1 mg/ml BSA.
Incubate at 37°C.
Storage Buffer
XbaI is supplied in:
10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 7 mM 2-mercaptoethanol, 1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage
After 50-fold overdigestion with XbaI, more than 95% of DNA fragments can be ligated and recut.
Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.
ER0681 |
1500 u (10 u/µl) |
1.00 ml |
ER0681 |
|
ER0682 |
5 x 1500 u (10 u/µl) |
2 x 1.00 ml |
ER0682 |
|
ER0683 |
HC, 7500 u (50 u/µl) |
2 x 1.00 ml |
ER0683 |
|
ER0685 |
3000 u (10 u/µl) |
2 x 1.00 ml |
ER0685 |
|
