产地：MBI
货号：ER0721
Conditions for 100% Activity
1X Buffer G:
10 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 50 mM NaCl and 0.1 mg/ml BSA.
Incubate at 55°C.
Storage Buffer
BclI is supplied in:
10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage
After 10-fold overdigestion with BclI, more than 95% of the DNA fragments can be ligated and recut.
| ER0721 | 1000 u (10 u/µl) | 1.00 ml | 1.00 ml | ER0721 | |
| ER0722 | 5000 u (10 u/µl) | 2 x 1.00 ml | 1.00 ml | ER0722 | |
| ER0725 | 3000 u (10 u/µl) | 2 x 1.00 ml | 1.00 ml | ER0725 |
