产地：MBI
货号：ER0821 Conditions for 100% Activity
1X Buffer B:
10 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2 and 0.1 mg/ml BSA.
Incubate at 37°C.
Storage Buffer
MboII is supplied in:
10 mM Tris-HCl (pH 7.5 at 25°C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage
After 5-fold overdigestion with MboII, more than 80% of the DNA fragments can be ligated in a reaction mixture containing 20-40 u of T4 DNA Ligase/1 µg of fragments and 10% PEG. More than 80% of these can be recut.
ER0821 |
300 u (5 u/µl) |
1.00 ml |
1.00 ml |
ER0821 |
|
ER0822 |
1500 u (5 u/µl) |
1.00 ml |
1.00 ml |
ER0822 |
|
