葡萄糖检测试剂 glucose assay reagent 50 mL 50 mL
蔗糖检测试剂 sucrose assay reagent 1 vial 1 小瓶
蔗糖标准品 sucrose standard 500 mg 500 mg
Description
Application This kit is for the quantitative, enzymatic determination of sucrose in food and other materials. Sucrose is hydrolyzed to glucose and fructose by invertase. Glucose and fructose are phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to sucrose concentration.
Properties
usage sufficient for 20 assays
storage temp. 2-8°C
References
reference Bergmeyer, H.U. and Bernt, E. Meth. Enzym. Anal. 2nd ed.,, 1176, (1974)
Printable Reviews
Components
Standard component
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