6xHis antibody
Cat.#: S2917
Rabbit Polyclonal Antibody
Clone ID: polyclonal
Swiss Prot: NA
Mol Weight: Size: 100ul
Recommended Applications
WB
Applications and Recommended Dilution Factors
WB: 1:500-1:2000
Products Data
Sample
A: 10 ug of non-transformed E. coli lysate
B: 10ug of non-transformed E. coli lysate, induced
C: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced
D: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced
12% SDS PAGE
diluated at 1:1000
Specificity
Rabbits were immunized with HHHHHH conjugated with KLH. After multiple immunizations in Freunds adjuvant serum was collected. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support
Storage Condition and Buffer
1X PBS/ 20% Glycerol/1% BSA (pH7.0). 0.01% Thimerosal was added as a preservative. Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Purified by antigen-affinity chromatography.
