| Catalogue Number | ANT-274 | ||||
| Synonyms | Integrin alpha-M, Cell surface glycoprotein MAC-1 subunit alpha, CR-3 alpha chain, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b antigen, ITGAM, CR3A, MO1A, CD11B, MAC-1, MAC1A, MGC117044. | ||||
| Type | Mouse Anti Human Monoclonal. | ||||
| Introduction | Integrins are heterodimeric integral membrane proteins consisting of an ? chain and a ? chain. CD11b is an I-domain including ? integrin combines with the ? 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor also called MAC1. The ? M beta 2 integrin is crucial in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. | ||||
| Clone | hCD11b. | ||||
| Immunogen | Purified human PBL Monocytes. | ||||
| Ig Subclass | Mouse IgG1. | ||||
| Purification Method | Ion exchange column. | ||||
| Protein concentration | 1mg/ml in PBS (after reconstitution). | ||||
| Storage Procedures | Lyophilized: store at 4°C. After reconstitution, if not intended for use within a month, aliquot and store at -20°C. | ||||
| Reconstitution | Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf LifeTwo years lyophilized, one month in solution at 4°C. | ||||
| Antibody Properties | Blocking and staining antibody. For staining, use 10µl/106 cells. Titer for blocking LPS binding should be determined by the investigator. | ||||
| Available Conjugates | This antibody is also available conjugated to biotin and FITC. For staining with biotin or FITC-conjugated antibody use 5-10µl/106 cells. | ||||
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | ||||
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