| Catalogue Number | ANT-251 | ||||
| Synonyms | Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein. | ||||
| Type | Mouse Anti Human Monoclonal. | ||||
| Introduction | CD14 (also known lipopolysaccharide (LPS) receptor) is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation. CD14 has been shown to bind apoptotic cells. | ||||
| Clone | hCD4. | ||||
| Immunogen | Purified human B Cells Ig SubclassMouse IgG1. | ||||
| Purification Method | Ion exchange column. | ||||
| Protein concentration | 1mg/ml in PBS (after reconstitution). | ||||
| Storage Procedures | Lyophilized: store at 4°C. After reconstitution, if not intended for use within a month, aliquot and store at -20°C. | ||||
| Reconstitution | Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf LifeTwo years lyophilized, one month in solution at 4°C. | ||||
| Antibody Properties | Blocking and staining antibody. For staining, use 10µl/106 cells. Titer for blocking LPS binding should be determined by the investigator. | ||||
| Available Conjugates | This antibody is also available conjugated to biotin and FITC. For staining with biotin or FITC-conjugated antibody use 5-10µl/106 cells. | ||||
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | ||||
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