| Catalogue Number | ANT-108 | |||||||
| Synonyms | BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra. | |||||||
| Type | Rat Anti Mouse Monoclonal. | |||||||
| Introduction | IL4 is a pleiotropic cytokine produced by activated T cells. IL4 is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. | |||||||
| Clone | NYRmIL-4. | |||||||
| Immunogen | r.Murine IL-4. | |||||||
| Ig Subclass | Rat IgG1. | |||||||
| Purification Method | Ion exchange. | |||||||
| Protein concentration | 1mg/ml in PBS (after reconstitution). | |||||||
| Shipping Conditions | Antibody is shipped lyophilized at ambient temperature. | |||||||
| Storage Procedures | In lyophilized form, for long periods, store at 4oC in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20oC. | |||||||
| Reconstitution | Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf LifeTwo years lyophilized, one month in solution at 4oC. | |||||||
| Applications | Direct ELISA, Western Blot, Immuneprecipitation, Immunohistochemistry, Intracellular staining. | |||||||
| Titer | By direct ELISA, 1:5,000 dilution will yield 0.4 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). | |||||||
| Usage | Prospec's products are furnished for LABORATORY ESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | |||||||
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