| Catalogue Number | ANT-296 |
| Type | Mouse Anti Human Monoclonal. |
| Introduction | MHC Class II is consists of 2 membrane spanning proteins. Each of these proteins is roughly 30 kDa, and has of two Globular domains, Alpha-1, Alpha-2, Beta-1 and Beta-2. The two regions farthest away from the membrane are alpha-1 and beta-1. The two proteins link without Covalent Bonds. The MHC Class II protein mainly presents peptides, which have been digested from external sources. MHC Class II is normally only expressed by Antigen Presenting Cells (APC) which digest foreign protein. Within the RER the alpha and beta proteins of the molecule, associate with each other, while a third protein called the "invariant chain," is necessary for stabilizing the complex, without it, they will not associate. The MHC-invariant complex is then passed from the RER, into and out of, the Golgi body. It fuses with an Endocytic compartment, where an external protein has been sampled and degraded. |
| Clone | CYR-hClass II. |
| Immunogen | Purified Human B cells. |
| Ig Subclass | Mouse IgG2a. |
| Specificity | All haplotypes. |
| Purification Method | Ion exchange column. |
| Protein concentration | 1mg/ml in PBS (after reconstitution). |
| Shipping conditions | Antibody is shipped lyophilized at ambient temperature. |
| Storage Procedures | Lyophilized: store at 4°C. After reconstitution, if not intended for use within a month, aliquot and store at -20°C. |
| Reconstitution | Reconstitute with sterile H20 to give a 1mg/ml concentration. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf LifeTwo years lyophilized, one month in solution at 4°C. |
| Titer | Staining antibody. For staining, use 10µl/106 cells. |
| Applications | FACS staining, Western Blot, Immuneprecipitation. |
| Available Conjugates | This antibody is currently available only as purified antibody. |
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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