Munktell Polyamide membrane filters:
Polyamide membranes are both hydrophilic and chemically resistant to most bases which makes them excellently suited as particle remover or sterilization filter for alcaline solutions.
Good compatibility with many solutions and aqueous samples makes them an economical choice for the clarification of the mobile phases for HPLC.
The high adsorption capacity and good mechanical strength make them useful for blotting techniques such as transfer and immobilization for nucleic acids.
They are not recommended as sterile filters for tissue culture solutions. Cellulose acetate membrane filters should be used for this application.
SPECIFICATION FOR POLYAMIDE MEMBRANE FILTERS
Adsorption: 100 μg/cm2 with bovine serum for 0.2 μm membranes.
Bubble Point (minimum values, wetted with water):
0.2 μm . . . . . . . . . . . . . 3.4 bar
0.45 μm . . . . . . . . . . . . . 2.2 bar
Flow Rates (minimum values per cm2 with water and △p = 1 bar (100 kPa)):
0.2 μm . . . . . . . . . . . . . 23 ml/min
0.45 μm . . . . . . . . . . . . . 46 ml/min
Compatibility:
compatible with solutions in pH range 3 – 14, resistant to some solvents.
Sterilization:
steam sterilization (121°C or 134°C), with ethylene oxide.
Thickness (average value):
125 μm
Validation:
Sterilization Capability of 0.2 μm membrane filter has been verified with
the BCT (Bacteria Challenge Test.