Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic RPMI 1640, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.
i Cells successfully cultured in Advanced RPMI 1640, with no adaptation, include Sp2, Vero, Raji, Daudi, and Jurkat.
This Advanced RPMI 1640 is manufactured as follows:
| With | Without |
| • Glucose | • L-glutamine |
| • Non Essential Amino Acids | • HEPES |
| • Sodium pyruvate | |
