Advanced MEM (Minimum Essential medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic MEM, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.
i Cells successfully cultured in Advanced DMEM ⁄ F-12, with no adaptation, include PK-15, MDCK, Vero, WI-38 and Hep-2.
This Advanced MEM is manufactured as follows:
| With | Without |
| • Glucose | • L-glutamine |
| • Non Essential Amino Acids | • HEPES |
| • Sodium pyruvate | |
