| Catalogue Number | ANT-209 | |||||
| Type | Rat Anti Mouse Monoclonal. | |||||
| Introduction | IL-12 is a heterodimeric cytokine that stimulates the production of interferon gamma from T-cells and natural killer cells, and also induces differentiation of Th1 helper cells. It is an initiator of cell-mediated immunity. | |||||
| Clone | NYRmIL-12p75. | |||||
| Immunogen | r.Murine IL-12 p75. | |||||
| Ig Subclass | Rat IgG2b. | |||||
| Purification Method | Ion exchange. | |||||
| Protein concentration | 1mg/ml in PBS (after reconstitution). | |||||
| Shipping Conditions | Antibody is shipped lyophilized at ambient temperature. | |||||
| Storage Procedures | In lyophilized form, for long periods, store at 4oC in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20oC. | |||||
| Reconstitution | Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf LifeTwo years lyophilized, one month in solution at 4oC. | |||||
| Applications | Direct ELISA, Western Blot, Immuneprecipitation. | |||||
| Titer | By direct ELISA, 1:5,000 dilution will yield 0.5 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). | |||||
| Note | This antibody recognizes the entire IL-12 molecule and has no reactivity to denatured p40 or p35. | |||||
| Usage | Prospec's products are furnished for LABORATORY ESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | |||||
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