Aldolase B(ALDOB)antibody
Cat.#: 2483-1
Rabbit Monoclonal Antibody
Clone ID: EPR3138Y
Swiss Prot: P05062
Mol Weight: 40kDa
Size: 100ul
Description
Aldolase B is one of three known aldolase isoenzymes, and is found in kidney and small adult intestine where it is associated with aldolases A or C. Aldolase B catalyzes the reversible cleavage of fructose 1-phosphate into dihydroxyacetone phosphate and glyceraldehyde. It is involved in both glycolytic and gluconeogenic pathways, where its quaternary structure is critical for maintaining its full catalytic function. Hereditary fructose intolerance (HFI), a potentially fatal human autosomal recessive disease of carbohydrate metabolism, results with Aldolase B deficiency. Partially active Aldolase B, however, has been reported in an HFI individuals, which supports the hypothesis that adequate gluconeogenesis/glycolysis is maintained in HFI patients by the presence of partially active enzymes (1-4).
Recommended Applications
WB, IHC, FC
Applications and Recommended Dilution Factors
WB: 1:500 - 1000
IHC: 1:100 - 250
FC: 1:20
Species Reactivity
Human, Mouse, Rat
Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only
Products Data
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Specificity
A synthetic peptide corresponding to residues in human Aldolase B was used as an immunogen.
Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Alternative Names
ALDOB antibody, ALDB antibody, ALDO2 antibody, Fructose-bisphosphate aldolase B antibody, Liver-type aldolase antibody
Description References
1. Rellos P, et al. J Biol Chem. 275(2):1145-51, 2000
2. Cross NC, et al. Cell. 53(6):881-5, 1988
3. Brooks CC, et al. FASEB J. 8(1):107-13, 1994
4. Gregori C, et al. J Biol Chem, 273(39):25237-25243, 1998
