| Catalogue Number | ANT-347 | ||
| Synonyms | HMG1, HMG3, SBP-1, Amphoterin, HMGB1, High-Mobility Group Box 1. | ||
| Type | Mouse Anti Human Monoclonal. | ||
| Introduction | High-mobility group box 1 protein (HMGB1), previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu.4, 5 Residues 27 - 43 and 178 - 184 contain a NLS. Posttranslational modifications of the molecule have been reported, with acetylation occurring on as many as 17 lysine residues. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins. | ||
| Clone | PJ2E1AT. | ||
| Immunogen | Anti-human HMGB1 mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human HMGB1 amino acids 1-215 purified from High Five (Trichoplusia ni) insect cells. | ||
| Ig Subclass | Mouse IgG2b heavy chain and κ light chain. | ||
| Purification Method | HMGB1 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. | ||
| Protein formulation | 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide. | ||
| Storage Procedures | For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles. | ||
| Stability / Shelf Life | 12 months at -20°C. 1 month at 4°C. | ||
| Applications | HMGB1 antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:500 ~ 1:2,000 and immunohistochemistry analysis is 1:100~300. Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:200. | ||
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | ||
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