Used as a coating material to support adherent cells growth and differentiation.
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.
Recommended for use as a cell culture substratum. May not be suitable for 3-D gel formation.
Sterile solution prepared from rat tail tendons by a modification of the published extraction method 1. It is supplied as an aqueous solution in 20mM acetic acid with a protein concentration as stated on the bottle. (approx.100mg protein per vial). Protein concentration was estimated by the Biuret method 2.
SDS polyacrylamide gel electrophoresis analysis shows the typical band pattern for Type I collagen, with a doublet at apparent molecular weights of 115 and 130kDa and another doublet at 215 and 235kDa. Based on this analysis, the purity of the collagen sample is >95%.
| Related Categories | 3D Printing Materials for Research and Development,Adhesion, Attachment, and Matrix Factors, Adhesion, Attachment, and Matrix Factors for Stem Cell Expansion, Attachment Factors, Attachments Factors, |
| description | Approx. 100mg/vial (Volume 22 - 25ml/vial, see label for lot-specific protein concentration) |
| sterility | sterile-filtered |
| product line | BioReagent |
| surface coverage | 6‑10 μg/cm2 |
| suitability | suitable for cell culture |
| storage temp. | 2-8°C |
| Gene Information | rat ... Col1a1(29393) |
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