| 应用 |
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. |
| 产品介绍 |
Alkaline phosphatase is a broad term associated with non-specific phosphomonoesterases with an alkaline pH optimum. |
| 备注 |
Chromatographically purified from P128630. Ribonuclease 0.002% by weight as RNase A using a poly C assay. Phosphodiesterase not detectable when assayed at 0.1 mg/ml with bis(p-nitrophenyl) phosphate. A suspension in 2.6M ammonium sulfate, pH 8.0. |
| 生化机理 |
Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters. |
| 别名 |
碱性磷酸酯酶Orthophosphoric-monoester phosphohydrolase (alkaline optimum);CIAP |
