| 应用 |
Papain is used as a meat tenderizer. It’s also used to break up tissue in the preparation of primary cell cultures. |
| 产品介绍 |
Papain is a sulfhydryl protease from Carica papaya latex. It has a molecular weight of 23 kDa and an optimum pH range of 6.0-7.0. The action of papain on leucine methyl ester produces an insoluble polyleucine peptide. Papain breaks down the intercellular matrix of cartilage. Papain is activated by cysteine, sulfide, and sulfite. Stabilizing agents are EDTA, cysteine and dimercaptoethanol. |
| 备注 |
Supplied as a two times crystalline suspension in 50mM sodium acetate, pH 4.5. To insure full activity, the enzyme should be incubated in a solution containing 1.1mM EDTA, 0.067mM mercaptoethanol and 5.5mM cysteine-HCl for 30 minutes. It is recommended that the enzyme be 0.22 micron filtered after dissolution and prior to use. |
| 生化机理 |
Papain is a cysteine protease that cleaves peptide bonds and is known to cleave the Fc portion of antibodies. |
| 别名 |
木瓜蛋白酶;番木瓜酶;木瓜朊酶;番瓜酵素;木瓜酶 |
