应用 |
DAB(3,3′-二氨基联苯胺)在许多应用中用于显示过氧化物酶活性。在过氧化物酶反应中,DAB在过氧化物存在下用作氢供体。DAB氧化形成不溶性棕色终产物,可用于免疫组织学和免疫印迹染色。 |
产品介绍 |
DAB (3-3′ diaminobenzidine tetrachloride) is suitable for immunoblotting and immunohistochemical staining of nucleic acids and proteins. In HRP-based assays, the buffer oxidizes to a brown polymer in the presence of HRP and hydrogen peroxide that is insoluble in most organic solvents; sites of HRP activity on tissue sections and blots appear as brown-orange deposits, which can be intensified by treatment with salts of silver, copper, nickel, cobalt and osmium. |
生化机理 |
3,3′-二氨基联苯胺四盐酸盐水合物已用于免疫组织染色、蛋白印迹和FISH(荧光原位杂交)。 |
别名 |
3,3'-二氨基联苯胺四盐酸盐,水合;[1,1'-联苯]-3,3',4,4'-四胺四盐酸盐水合物;3,3'-二氨基联苯胺四盐酸盐(DAB)(水合物);DAB·4HCl Hydrate;
3,3',4,4'-Tetraaminobiphenyl Tetrahydrochloride Hydrate;
3,3',4,4'-Biphenyltetramine tetrahydrochloride |