| 应用 |
色度法检测各种脱氢酶的电子受体（Electron acceptor）碘硝基四唑紫已用于细胞染色。它已被用作 D-阿拉伯糖醇的酶促测定中的偶联剂。 |
| 产品介绍 |
Iodonitrotetrazolium chloride is an electron acceptor used in various colorimetric assays. The chemical has been mainly documented for its measurement of dehydrogenase activity. In the assay to measure SDH (succinate dehydrogenase) activity, Iodonitrotetrazolium chloride was used as an oxidizing agent being reduced by succinate dehydrogenase. Upon reduction the compound forms a formazan dye measured at 500 nm. Iodonitrotetrazolium chloride has also been used to study the electron transport activity in soils, sediments, and pure enzyme cultures as well as nucleotide diphosphate kinase activity. |
| 生化机理 |
spectrophotometric assay for succinate dehydrogenase activity: Materials: spectrophotometer cuvette with 1-cm light patch. Reaction Mixture: 100 mmol/liter triethanolamine (HCl), pH8,3 o.5 mmol/liter EDTA, 2 mmol/liter Iodonitrotetrazolium chloride, 12 g/liter Cremophor EL, 20 mmol/liter succinate, pH7.4 Reaction: mix 990 ul of reaction mixture with 10ul mitochondrial suspension color develops at 30oC record for 6 min. @500 nm |
| 别名 |
碘硝基氯化四氮唑蓝;2-(对碘苯基)-3-(对硝基苯基)-5-苯基四唑氯水合物;2-(4-碘苯基)-3-(4-硝基苯基)-5-苯基氯化四唑氯水合物;碘硝基氯化四氮唑;INT;对碘硝基四唑紫;2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride;
p-Iodonitrotetrazolium Violet;
INT
Iodonitrotetrazolium chloride |
