| 应用 |
微生物学检验试剂，用于细胞色素氧化酶阳性好氧微生物的分类；对于氧化酶试剂，使用 1% 的 N,N,N′,N′-四甲基对苯二胺二盐酸盐的水溶液 |
| 产品介绍 |
N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride(TMPD) is an oxidizable compound that serves as a reducing co-substrate for heme peroxidases. TMPD undergoes one-electron oxidation by the heme peroxidase higher oxidation states (compounds I and II) producing a highly colored product that absorbs at 611 nm. Thus, the stoichiometry of oxidation is 2 moles of TMPD oxidized per mole of hydroperoxide reduced by the peroxidase. The extinction coefficient of the oxidized TMPD at 611 nm is 12,200.2 TMPD is also used for the detection of peroxidases on polyacrylamide gels. |
| 别名 |
N,N,N',N'-四甲基-1,4-苯二胺二盐酸盐; Wurster 试剂; TMPPD;N,N,N',N'-Tetramethyl-1,4-phenylenediamine Dihydrochloride; Wurster’s reagent; 1,4-Bis(dimethylamino)benzene dihydrochloride; Wurster's Blue dihydrochloride |
