Acclaim®120 C8 reversed-phase columns feature a densely-bonded monolayer of octyldimethylsiloxane on a highly pure, spherical silica substrate with a 120 Å pore structure. The columns are a well-characterized line of LC/MS-compatible C8 phases with very high surface coverage and very low metal content. These columns provide exceptional performance for a variety of applications in the pharmaceutical, nutraceutical, food, and life sciences.
Ultrapure silica with high-density bonding for symmetrical peak shapes
High hydrophobicity, low polarity phases for predictable reversed-phase separations
Acclaim 120 C8 column have high surface coverage, resulting in high-capacity columns. They are stable between pH 2 and 8 and are available in 3 and 5µm particle sizes, and 4.6, 3.0, and 2.1 mm-diameters, with an average pore diameter of 120Å. Acclaim 120 C8 is also available in 2.2µm RSLC format (see Acclaim RApid Separation LC Columns section for more details). All phases are LC/MS compatible because of the low silica bleed that results from the stable bonding procedure.
| Acclaim 120 Silica-Based Reversed-Phase Columns for Small Molecule AnalysisSpecifications | |
|---|---|
| Particle shape | Spherical |
| Particle size distribution (40/90) | 1.2 |
| Total carbon content | C18: 18% C8: 11.2% |
| Carbon surface coverage | C18: 3.2 μmol/m2 C8: 3.7 μmol/m2 |
| Pore volume | 0.9 mL/g |
| Average pore diameter | 120Å |
| Surface area | 300 m2/g |
| pH Range | 2–8 |
| Temperature | <60°C |
| End-capped | Yes |
| Metal impurity (Na, Fe, Al) | <10.0 ppm Total Metal <3.0 ppm Individual Metal |
| USP listing | C18: L1 C8: L7 |
| Starting material | Ultrapure silica |
| Particle size | 3, 5µ |
Isocratic separation of six cardiac antiarrhythmic drugs (Beta-Blockers).
Loperamide in anti-diarrhea tablets.
Fluticasone in serum.
Carbamate insecticides in EPA 531.1 with MS detection.
| Analytical Columns | |
|---|---|
| Acclaim 120, C8, 3 µm Analytical (2.1 x 50 mm) | 059122 |
| Acclaim 120, C8, 3 µm Analytical (2.1 x 100 mm) | 059123 |
| Acclaim 120, C8, 3 µm Analytical (2.1 x 150 mm) | 059124 |
| Acclaim 120, C8, 3µm Analytical (2.1 x 250mm) | 076185 |
| Acclaim 120, C8, 3µm Analytical (3.0 x 150 mm) | 068970 |
| Acclaim 120, C8, 3µm Analytical (3.0 x 100mm) | 076184 |
| Acclaim 120,C8, 3 µm, Analytical,(3.0 x 250 mm) | 070078 |
| Acclaim 120, C8, 3 µm Analytical (4.6 x 50 mm) | 059125 |
| Acclaim 120, C8, 3 µm Analytical (4.6 x 100 mm) | 059126 |
| Acclaim 120, C8, 3 µm Analytical (4.6 x 150 mm) | 059127 |
| Acclaim 120, C8, 5 µm Analytical (2.1 x 50 mm) | 059134 |
| Acclaim 120, C8, 5 µm Analytical (2.1 x 100 mm) | 059135 |
| Acclaim 120, C8, 5 µm Analytical (2.1 x 150 mm) | 059136 |
| Acclaim 120, C8, 5 µm Analytical (2.1 x 250 mm) | 059137 |
| Acclaim 120, C8, 5 µm Analytical (4.6 x 50 mm) | 059138 |
| Acclaim 120, C8, 5 µm Analytical (4.6 x 100 mm) | 059139 |
| Acclaim 120, C8, 5 µm Analytical (4.6 x 150 mm) | 059140 |
| Acclaim 120, C8, 5 µm Analytical (4.6 x 250 mm) | 059141 |
| Guard Columns | |
|---|---|
| Acclaim 120, C8, 5 µm Guard Cartridges, (4.6 x 10 mm), 2 ea (use V-2 Holder) | 069696 |
| Acclaim 120 C8, 5µm Guard Cartridges (3 x 10mm), 2ea, requires holder 069580 | 071979 |
| Acclaim 120, C8, 5µm, Guard Cartridges, (2.1 x 10mm), 2 ea; requires holder 069580 | 069688 |
| Acclaim SST Guard Cartridge Holder V-2 | 069580 |
| Acclaim Guard Kit (Holder and coupler) V-2 | 069707 |
| Guard to Analytical Column Coupler V-2 | 074188 |
| Acclaim RSLC 120 C8 Analytical Columns | |
|---|---|
| Acclaim RSLC C8, 2.2 µm Analytical (2.1 x 30 mm) | 072614 |
| Acclaim RSLC 120, C8, 2.2µm, 2.1 x 50 mm | 072615 |
| Acclaim RSLC 120, C8, 2.2µm, 2.1 x 100 mm | 072616 |
| Acclaim RSLC 120, C8, 2.2µm, 2.1 x 150 mm | 072617 |
| Acclaim RSLC 120, C8, 2.2µm Analytical (2.1 x 250mm) | 074811 |
| Acclaim RSLC 120, C8, 2.2µm, 3 x 30mm | 072618 |
| Acclaim RSLC 120, C8, 2.2µm, 3 x 50mm | 072619 |
| Acclaim RSLC 120, C8, 2.2µm, 3 x 100mm | 072620 |
| Micro and Nano Columns | |
|---|---|
| Acclaim PepMap100, C8, 5 µm, 100 Å, 75 µm i.d. x 5 cm | 161555 |
| 75 µm i.d. x 15 cm, packed with Acclaim PepMap100 C8, 3 µm, 100Å, nanoViper | 164706 |
| 300 µm i.d. x 15 cm, packed with Acclaim PepMap100 C8, 3 µm, 100Å, nanoViper | 164722 |
