Acclaim®PepMap300 C18 is used for the separation of larger peptides and proteins. It is available in 5 µm particle sizes, with 300 Å pore sizes, and in nano, capillary, and micro formats.
| Acclaim PepMap for TFA-Free Peptide Mapping Specifications | |||
|---|---|---|---|
| Column type | Nano | Capillary | Micro |
| Column i.d. | 75 µm | 300 µm | 1.0 mm |
| Length | 50, 150, 250, and 500 mm | 50, 150, and 250 mm | 50, 150, and 250 mm |
| Recommended Flow Rate | 200 nL/min | 4 µL/min | 40 µL/min |
| Packing Material | C18, 100 Å, 3 µm | C18, 100 Å, 3 µm | C18, 100 Å, 3 µm |
| C18, 300 Å, 5 µm | C18, 300 Å, 5 µm | C18, 300 Å, 5 µm | |
| C8, 100 Å, 3 µm | C8, 100 Å, 3 µm | C8, 100 Å, 3 µm | |
| C8, 100 Å, 5 µm | C8, 100 Å, 5 µm | C8, 100 Å, 5 µm | |
| C4, 300 Å, 5 µm | C4, 300 Å, 5 µm | C4, 300 Å, 5 µm | |
| Column | Fused-silica | Glass Lined Tubing | Stainless steel |
| Column Inlet | 1/16-in. PEEK sleeve adapter (provided) nanoViper (optional) | 1/16-in. universal fitting nanoViper (optional) 50µm i.d. PEEK sheathed fused silica with nanoViper fittings | 1/16-in. PEEK tubing nanoViper (optional) 75µm i.d. PEEK sheathed fused silica with nanoViper fittings |
| Column Outlet | Fused-silica 25-µm i.d./ 280-µm o.d. | Fused-silica 75-µm i.d./ 280-µm o.d | 1/16-in. PEEK tubing nanoViper (optional) 75µm i.d. PEEK sheathed fused silica with nanoViper fittings |
| MS Outlet | 1-m Length | ||
| Nano LC Columns | |
|---|---|
| Acclaim PepMap100, C18, 3 μm, 100 Å, 75 μm i.d. × 5 cm, nanoViper | 164567 |
| Acclaim PepMap300, C18, 5 µm, 300 Å Wide Pore, 75 µm x 15 cm | 163574 |
| Capillary LC Columns | |
|---|---|
| Acclaim PepMap300, C18, 5 µm, 300 Å Wide Pore, 300 µm x 5 cm | 163580 |
| 300 µm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 µm, 300Å, nanoViper | 164718 |
| Micro LC Columns | |
|---|---|
| 1.0 mm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 µm, 300Å, nanoViper | 164719 |
| 1.0 mm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 µm, 300Å, nanoViper | 164719 |
