A variety of HPLC approaches are proposed for characterizing oligosaccharides, but many prove inadequate for separating complex mixtures and are limited by a lack of specificity and high limits of detection. Dionex has developed an improved HPLC technique for carbohydrate analysis based on anion-exchange chromatography coupled with pulsed amperometric detection. This technique provides high-resolution separations with sensitive and specific detection, without derivatization.
Sensitive, direct detection with no derivatization, using pulsed amperometric detection
Very high-efficiency separations
Separations based on size, charge, degree of branching, and linkage isomerism
Low flow rate saves on eluent consumption
Does not require derivatization
CarboPac®PA200 columns are packed with a hydrophobic, polymeric, pellicular anion-exchange resin stable over the range of pH 0–14. The unique pH-stability of the packing material allows the use of eluent compositions that are conducive to anodic oxidation of carbohydrates at gold electrodes.
| CarboPac PA200 Column for High-Resolution Oligosaccharide Separations Specifications | |
|---|---|
| Dimensions | CarboPac®PA200 Analytical Column: 3×250 mm CarboPac PA200 Guard Column: 3×50 mm |
| Maximum operating pressure | 4000 psi (27.9 MPa) |
| Temperature range | 6–60°C Recommended operating temperature: controlled ambient |
| Typical operating conditions | 2700 psi at 0.5 mL/min (analytical and guard columns) Recommended flow rate: 0.5 mL/min |
| Mobile phase compatibility | pH 0–14; 100% compatible with common organic solvents. Ionic form eluents: sodium acetate and sodium hydroxide only. |
| Resin composition | 5.5 µm-diameter ethylvinylbenzene/divinylbenzene substrate (55% cross-linked) agglomerated with 43 nm MicroBead®quaternary amine functionalized latex (6% crosslinked) |
| Anion-exchange capacity | Approximately 35µeq/column (3×250 mm analytical column) |
| Column construction | PEEK™with 10-32 threaded ferrule-style end fittings. All components are nonmetallic |
The CarboPac PA200 column is the latest addition to the CarboPac family of columns for carbohydrate separations. This column has been specially developed to provide high resolution separations of charged and neutral oligosaccharides and is the recommended column for these applications. The CarboPac PA200 columns are packed with a hydrophobic, polymeric, pellicular anion exchange resin stable over the range of pH 0–14. This unique pH-stability of the packing material allows the use of eluent compositions that are conducive to anodic oxidation of carbohydrates at gold electrodes.
Resin Characteristics:
Particle Size:5.5 µm
Pore Size: microporous (< 10 Å)
Cross-linking:55%
Ion exchange capacity: 35 µeq per column
Latex Characteristics:
Functional Group:quaternary ammonium ion
Latex Diameter: 43 nm
Latex Cross-linking: 6%
Typical Operating Parameters:
pH range: 0–14
Temperature Limit: 4–60 °C
Pressure Limit:3500 psi
Organic Solvent Limit: 100% compatible
Typical eluents: High purity water (18.2 megohm-cm), sodium hydroxide, sodium acetate
| Guard Columns | |
|---|---|
| CarboPac PA200 Guard Column (3 x 50 mm) | 062895 |
| Analytical Columns | |
|---|---|
| CarboPac PA200 Analytical Column (3 x 250 mm) | 062896 |
