Dionex CarboPac SA10-4µm Column for Fast, High-Resolution Mono- and Disaccharide Analysis
The New Thermo Scientific™Dionex™CarboPac™SA10-4µm Column is designed for fast chromatographic analysis of simple sugars in Biofuel and Food samples. The peak efficiencies produced by the Dionex CarboPac SA10-4µm columns produce 60 – 80% higher peak efficiencies than standard Dionex CarboPac SA10 columns, thus producing better resolution and easier peak identification and integration. The Dionex CarboPac SA10-4µm column can analyze six common sugars in Food samples in only 10 min, or eight common Biofuel mono- and disaccharides in less than 8 min.
This is the first in a series of Dionex CarboPac columns that use smaller resin particles. The smaller particles produce more efficient peaks and better resolution making analysis easier and more reliable. The smaller particles also produce higher system back pressure (over 3000 psi) under standard operating conditions and therefore can only be run using Thermo Scientific™Dionex™HPIC™systems such as theThermo Scientific Dionex ICS-5000+HPIC system, which can operate up to 5000 psi.
The Dionex CarboPac SA10-4µm column should be used as a direct replacement for the Dionex CarboPac SA10 column only if an HPIC system is available
The polymeric Dionex CarboPac SA10-4µm column is packed with 4 µm supermacroporous substrate, coated with the same strong anion-exchange layer of latex nano beads as the Dionex CarboPac SA10 column, which provides high capacity and rapid, high-resolution separations. The Dionex CarboPac SA10-4µm columns feature high reproducibility, and can withstand thousands of injection cycles under high pH conditions.
| Thermo Scientific™Dionex™CarboPac™SA10-4µm Column for Monosaccharide and Disaccharide AnalysisSpecifications | |
|---|---|
| Dimensions | Dionex CarboPac SA10-4µm Analytical Column: 4×250 mm and 2 × 250 mm Dionex CarboPac SA10-4µm Guard Column: 4 × 50 mm and 2 × 50 mm |
| Maximum operating pressure | 5,000 psi (34.5 MPa) |
| Temperature range | 4–60 °C |
| Typical operating conditions | 4,000 psi at 1.5 mL/min (standard-bore column) or 0.38 mL/min (microbore column) Recommended flow rate: 1.5 mL/min (standard-bore) or 0.38 mL/min (microbore) |
| Typical Eluents | Potassium hydroxide via Eluent Generation |
| Resin composition | 4-µm diameter sumpermacroporous substrate (ethylvinylbenzene 55% crosslinked with divinylbenzene) agglomerated with 55-nm MicroBead difunctional quaternary ammonium ion (4.5% cross-linked). |
| Anion-exchange capacity | Approximately 290 µeq/column (4×250 mm standard-bore column) Approximately 73 µeq/column (2 × 250 mm microbore column) |
| Column construction | PEEK |
Fast separation of biofuel sugars on the Dionex CarboPac SA10-4μm column.
Fast separation of food sugars on the Dionex CarboPac SA10-4μm column.
High-resolution separation of a high concentration corn stover hydrolysate sample.
Comparison of Dionex CarboPac SA10 (2 mm) and Dionex CarboPac SA10-4μm (2 mm) for the analysis of biofuel sugars.
| Analytical Columns | |
|---|---|
| Dionex CarboPac SA10-4µm Analytical Column (4 x 250 mm) | 088233 |
| Dionex CarboPac SA10-4µm Analytical Column (2 x 250 mm) | 088235 |
| Guard Columns | |
|---|---|
| Dionex CarboPac SA10-4µm Guard Column (4 x 50 mm) | 088234 |
| Dionex CarboPac SA10-4µm Guard Column (2 x 50 mm) | 088236 |
