Hydrophobic Interaction Chromatography separates biomolecules in a decreasing salt gradient, based on differences in surface hydrophobicity. This method preserves the biological activity of proteins. The HIC separation mechanism is complementary to those of ion-exchange and gel filtration chromatography.
Based on 5 µm ultrahigh purity spherical silica gel particles, with 300 Å pores
Proteins separated under non-denaturing conditions
High protein loading capacity for protein purification applications
Proprietary multidentate silica bonding for enhanced hydrolytic stability
High-resolution HPLC separation of proteins and peptides
Wide range of applications
The ProPac®HIC-10 column is a high-resolution, high-capacity, silica-based HIC column that provides greater hydrolytic stability under the highly aqueous conditions used in HIC. Examples include the separation of bovine serum proteins, snake venom proteins, enzymes, human skeletal muscle protein (HSMP), monoclonal antibodies, pancreatin, and thrombin and peptide applications including tryptic digests of proteins.
| ProPac HIC ColumnsSpecifications | |
|---|---|
| Protein Loading Capacity | 340 mg Lysozyme per 7.8 x 75 mm column |
| Starting Material | Ultrapure Spherical Silica |
| Particle Size | 5µm |
| Pressure Limit | 4,000 psi |
| Column Construction | Stainless Steel |
| Phase | HIC |
| Average Pore Diameter | 300Å |
| Endcapped | No |
| Mobile Phase Compatibility | 2 M Ammonium sulfate/ phosphate salts, organic solvents for cleanup |
| pH range | pH 2.5–7.5 |
| Temperature Limit | < 40°C |
Separation of a mixture of proteins using the ProPac HIC-10, compared to the same separation on two competitors’ columns.
Comparison of the separation of snake venom proteins/peptides using the ProPac HIC-10 and two competitors’ columns.
Gradient separation of a monoclonal antibody using the ProPac HIC-10.
| Analytical Columns | |
|---|---|
| ProPac HIC-10 Column (2.1 x 100 mm) | 063653 |
| ProPac HIC-10 Column (4.6 x 100 mm) | 063655 |
| ProPac HIC-10 (4.6 x 250mm) | 074197 |
| ProPac HIC-10 Column (7.8 x 75 mm) | 063665 |
