产地：MBI
货号：EN0361
Applications
Control of carry-over contamination in PCR (2).
Glycosylase mediated single nucleotide polymorphism detection (GMPD) (3).
Site-directed mutagenesis (4).
As a probe for protein-DNA interaction studies (5).
SNP genotyping.
Cloning of PCR products (6).
Generation of single strand overhangs of PCR products and cDNA.
Description
Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar, leaving an apyrimidinic site in uracil-containing single or double-stranded DNA, see Fig.1. Shows no activity on RNA (1).
