MAbPac HIC–Butyl for Monoclonal Antibody (mAb) Analysis
The Thermo Scientific™ MAbPac™ HIC-Butyl column is a high-resolution, polymer-based hydrophobic interaction chromatography (HIC) column designed for the separation of mAbs and antibody-drug conjugates (ADCs). The hydrophilic resin and optimal density of the butyl groups lead to excellent biocompatibility and low carryover.
Product Highlights:
• Optimal selectivity for ADCs
• High resolution and high efficiency
• Stable at wide range of pH (2-12)
• Low carryover
• Rugged column packing
The MAbPac HIC-Butyl column is based on hydrophilic, nonporous, 5 µm polymer resin functionalized with butyl groups. The MAbPac HIC-Butyl column provides complementary selectivity to MAbPac HIC-10 and MAbPac HIC-20 columns.
| MAbPac™HIC-Butyl Monoclonal Antibody Analysis Column Specifications | |
|---|---|
| Column Dimension | 4.6 × 100 mm 4.6 x 10mm guard cartridges |
| Particle Size | 5 μm |
| Bonding Chemistry | Butyl |
| Silica Substrate | Polymer |
| Pore size | Non-porous |
| Flow Rate Range: | 0.4 to 1.0 mL/min. |
| Pressure Limit | 4,000 psi for 4.6 x 100mm column 4,000 psi for 10mm guard cartridges |
| Temperature Range: | 10 to 60 ºC |
| pH Range | 2.0 - 12.0 |
| Typical buffers: | Mobile phase formula 1 Mobile phase formula 2 |
| Solvent Compatibility: | Compatible with 50% organic solvents |
HIC is widely used as an orthogonal method to cation-exchange chromatography (CEX) and size-exclusion chromatography (SEC) for the characterization of mAbs. HIC separates proteins based on the reversible interaction with the hydrophobic stationary phase. Proteins bind to the weakly hydrophobic stationary phase in the presence of high salt concentration and elute off the column as the concentration of salt decreases. In contrast to reversed-phase liquid chromatography, HIC typically preserves the biological activity of the protein, which is useful for downstream functional analysis such as binding and cell-based potency assays. In addition, HIC provides separation with little carryover.
The MAbPac HIC-Butyl column is a high resolution polymer based HIC column designed for the separation of mAbs and ADCs. The hydrophilic resin and optimal density of the butyl groups lead to excellent bio-compatibility and low carryover.
| Analytical Columns | |
|---|---|
| MAbPac HIC-Butyl, 5µm, Analytical Column, 4.6 x 100 mm | 088558 |
| Guard Columns | |
|---|---|
| MAbPac HIC-Butyl, Guard Cartridges 5 µm, 4.6 x 10 mm (2/pk) | 088559 |
| Acclaim SST Guard Cartridge Holder V-2 | 069580 |
| Acclaim Guard Kit (Holder and coupler) V-2 | 069707 |
| Guard to Analytical Column Coupler V-2 | 074188 |
