Nanobore column chromatography has become a method of choice when analyzing a wide variety of peptide and protein samples. The complex nature of biological samples requires frequent testing of different separation methods on a variety of nanobore columns to determine the best separation conditions. Here we test a newly developed easy-to-use chip based system enabled with different column lengths, column heating and universal connectivity. The flexible design of the chip columns facilitates method development, providing columns with different inner diameters, bed lengths and a wide selection of stationary phase materials. The PicoChip® column has sheath gas capability which further expands the possible use of PicoChip columns for higher flow rates (> 1 μL/min.) and on different MS platforms (data not included).