实验方法
实验方法

ACIDIC-NATIVE GEL Protocol( For Basic Proteins (pI7.0))

来源： 互联网 关键词： acidic-native acidic

 

USE ONLY FRESH GELS

Stock Solutions

1) 1.5M Acetate-KOH pH 4.3 (48ml 1MKOH + 17.2ml AcH + H2O up to 200ml) - Keep RT.
2) 30% Acrylamide 0.8% Methylene bis Acrylamide. Keep 4°C.
3) 0.25M Acetate-KOH pH 6.8 (48ml 1MKOH + 2.9ml AcH + H2O up to 200ml) - Keep RT.
4) 10% Ammonium Persulfate (APS). Keep 4°C less than 1 month.


Running Buffer x1
 
ß-Alanine
0.35M
18.7gr
AcH
0.14M 
4.8ml 
H2O up to
  
600ml
Adjust pH to
  
4.3
Keep at RT


Dissolving Buffer x5
 

Glycerol 50%
1.45ml
0.25M Acetate-KOH pH 6.8 
0.5ml
Methyl Green
traces

 

Keep in aliquots of 1ml at -20C
 
 

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