Preparation of Brain Membrane Fractions
Materials Ready Gel Precast Gels Bio-Rad Mini-Protean-3 Cell apparatus Prestained protein markers Reagents 6x Laemmli reducing sample buffer Distilled H20 (dH20) Running buffer Cold Transfer buffer Before you start: Turn on heat block to 95-100°C Make sure ice block cooling unit is prepared for transfer Procedure 1
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