The manuals for this section have been compiled by Susan L. Wall, HT(ASCP). These manuals are in Adobe Acrobat PDF format and require the Acrobat Reader for viewing.

Staining Manuals

• Fixative Solutions: Each of the following .PDF files contains the procedure plus a sample container label.

• 95% Alcoholic Bouin's Fixative

• 95% Alcohol Fixative

• B5 Fixative

• Bouin's Fixative

• Formalin Fixative

• Karnovsky's Fixative (glutaraldehyde)

• Hartman's Fixative

• Hollande's Fixative

• Orth's Solution (dichromate fixative)

• Zenker's Fixative

• Stock Solutions: Each of the following .PDF files contains the information regarding the use and preparation of a working solution.

• Acid cleaning solution for glassware

• Bluing solution for hematoxylin stain

• Celloidin slide adhesive

• Chrome alum adhesive

• Glycerin water mounting medium

• H and E acid decolorizing rinse

• Hypo (sodium thiosulfate)

• Lugol's iodine for mercury removal

• Metal embedding mold release

• Paraffin block softening solution

  
  

• Miscellaneous Labels

• Staining Procedures: Each of the following .PDF files contains the procedure for a special stain, a procedure card, and sample container labels for the reagents.

• Introduction

  
  

• Acid Fast Stain (for mycobacteria)

• Acid Fast Stain (Ziehl-Neelsen, for M. leprae and Nocardia)[contributed by Greg Winn, Specialist Histopathology & Cytology Services, Western Australia]

• Fite's Acid Fast Stain

• Alcian Blue Stain

• Alcian Blue-PAS Stain (PAB)

• Hyaluronidase Digestion for Alcian Blue

• Alizarin Stain for calcium

• Auramine-Rhodamine Stain (fluorescent)

• Bielschowsky Stain (for senile plaques)

• Bile Stain

• Bodian's Stain

• Colloidal Iron Stain

• Congo Red Stain

• Copper Stain

• Elastic van Gieson Stain

• Elastic - Weigert's resorcin-fuchsin method

• Modified Elastic van Gieson Stain

• Fontana-Masson Stain for melanin

• Melanin Bleach

• Fraser Lendrum Stain

• Giemsa (Modified May-Gruenwald) Stain (for hematopoietic tissues)

• Giemsa Stain (for Helicobacter)

• Gram (Modified Brown-Brenn) Stain

• Gridley's Stain for ameba

• Grimelius Argyrophil Stain (Pascual's Method)

• Grocott's Methenamine Silver (GMS) Stain

• Holzer's Glial Fiber Stain

• Hortega's Pineal Stain

• Iron Stain (Prussian blue)

• Iron Stain (Turnbull's blue)

• Jones' Silver Stain

• Luxol Fast Blue (LFB) Stain

• Methyl Green Pyronin (MGP) Stain

• Mucicarmine Stain

• Nissl Stain

• Oil Red O Stain

• Orcein Stain

• Periodic acid-Schiff Stain (PAS)

• Periodic acid-Schiff, digested Stain (PAS-D)

• PTAH Stain

• Reticulin Stain

• Spirochete Stain (Steiner & Steiner method)

• Sudan Black B Stain (for lipochrome)

• Sudan Black B Stain (for fat)

• Trichrome Stain - Masson's method

• Trichrome Stain - microwave method

• Thioflavin S Stain (for amyloid in tissues)

• Modified Thioflavin S Stain (for senile plaques)

• Toluidine Blue Stain (for mast cells)

• Urate Crystal Stain

• VonKossa Stain for calcium

Safety Procedures

• Bloodborne Pathogens (Exposure Control Plan)

• Working with Chemicals

• Chemical Hygiene Safety

• Chemical Inventory

• Emergency Procedures

• Facilities (engineering controls)

• Safety Equipment (engineering controls)

• Training Records

• Tuberculosis Guidelines