Cells are grown for 2-3 days as 1.5ml prep. under selection for the plasmid of interest. Spin cells down 2.6K for 5min.
Resuspend in 1ml 0.25m NaOH/1% 2-mercaptoethanol
Incubate 10min on ice.
Add 0.16ml 50% trichloroacetic acid
Incubate for 10min on ice.
Pellet 14K 10min.
Resuspend pellet in 1ml ice cold acetone.
Pellet 14K 10min.
Air dry pellet and resuspend in 200-500ul SDS SB and proceed as described below in section on western transfer