这一技术最初主要用于材料、冶金、地质、矿物等研究。CEITEC欧洲工程技术中心AtomTrace LIBS研究团队可以说是最早关注到LIBS技术在生命科学和生物医学领域应用的科研团队之一。2005年，Jozef Kaiser博士（Atomtrace科学主任、布尔诺大学教授、激光光谱学研究室负责人、CEITEC物质特性与表面科学研究部主任）等在European Physical Journal上发表了“Mapping of the metal intake in plants by large-field X-ray microradiography and preliminary feasibility studies in microtomography”(Eur.Phys.J. D 32, 113-118)；2006年又利用LIBS飞秒激光光谱分析技术研究分析了植物样品铁、锰元素的分布并首次作出完整叶片铁元素的二维分布图，并发表了“Femtosecond laser spectrochemical analysis of plant samples”（Laser Phys.Lett.3, No.1, 21-25, 2006）。

2007年至2010年，以Kaiser博士为代表的研究团队利用μCT与飞秒激光诱导击穿光谱技术（LIBS）及LA-LCP-MS等技术，对向日葵等植物组织中重金属元素吸收积累进行了研究分析，先后发表论文：

1)Monitoring of the heavy-metal hyperaccumulation in vegetal tissues by X-ray radiography and by Femto-second laser induced breakdown spectroscopy. Microscopy research and technique 70:147-153, 2007；

2)Utilization of laser induced breakdown spectroscopy for investigation of the metal accumulation in vegetal tissues. SpectrochimicaActa Part B 62:1597-1605；

3)Investigation of heavy-metal accumulation in selected plant samples using laser induced breakdown spectroscopy and laser ablation inductively coupled plasma mass spectrometry. Appl. Phys. A 93:917-922, 2008；

4)Multi-instrumental analysis of tissues of Sunflower plants treated with Silver(I) ions—plants as bioindicators of environmental pollution

5)Mapping of lead, magnesium and copper accumulation in plant tissues by laser-induced breakdown spectroscopy and laser-ablation inductively coupled plasma mass spectrometry. SpectrochimicaActa Part B 64:67-73, 2009;

6)Sunflower plants as bioindicators of environmental pollution with lead(II) ions. Sensors 9:5040-5058, 2009；

7)Detection of lead in Zea mays by dual-energy X-ray Microtomography at the SYRMEP Beamline of the ELETTRA Synchrotron and by Atomic Absorption Spectroscopy. Microscopy Research and Technique 73:638-649, 2010;

8)Determination of plant thiols by liquid Chromatography Coupled with Coulometric and Amperometric Detection in Lettuce treated by lead(II) ions. Electroanalysis 22 No.11:1248-1259, 2010

在这些研究中，CEITEC/Atomtrace LIBS研究团队不仅最早利用LIBS技术作出完整叶片元素分布图，还最早利用LIBS技术与显微CT（μCT）相结合进行三维化学成像构建，同时还开展了小鼠肾脏、蛇骨、古生物牙齿等动物样品的LIBS元素分析研究。

2012年，Kaiser博士等根据多年的研究成果和同行研究情况，撰写发表了综述性文章“Trace elemental analysis by Laser-induced breakdown spectroscopy---biological applications”(Surface Science Report 67:233-243, 2012)。上述研究成果奠定了以Kaiser博士等为代表的激光光谱学实验室LIBS技术在全球生命与环境科学应用领域的领先地位。

在研究植物养分利用与重金属毒害时，LIBS技术经常会与叶绿素荧光技术联合应用。2012年，CEITEC激光光谱学实验室研究团队与PSI公司合作，在《SpectrochimicaActa Part B》发表了“Application of laser-induced breakdown spectroscopy to the analysis of algal biomass for induxrial biotechnology”,该研究利用LIBS技术和特制液体样品激光作用室，分析了藻类培养液与生物膜中钾、镁、钙、钠等与藻类至关重要的元素及铜等毒性重金属元素积累。

印度阿拉哈巴德大学则通过FP100叶绿素荧光仪（FluorCam的非成像版）和LIBS技术结合，研究了小麦经过重金属处理，植物体内元素含量变化与叶绿素荧光光合特性的关系。研究成果连续发表了多篇文献（Tripathi，2016；Tripathi，2015）

  
      图18. 左：LIBS藻类测量方案；右：藻类生物膜脉冲激光剥蚀孔

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易科泰生态技术致力于引进、消化、吸收和创新国际先进生物生态科研技术，提供植物表型分析、作物胁迫敏感性与抗性检测、光养生物反应器／藻类培养与在线监测、生态毒理学检测技术方案和实验服务与合作。旗下的Ecolab实验室目前配备有FluorCam封闭式荧光成像系统、FluorCam封闭式荧光成像系统、FluorCam便携式荧光成像仪、FL3500叶绿素荧光仪、FluorPen手持式叶绿素荧光仪、AquaPen藻类荧光仪、PolyPen手持式植物光谱测量仪、SpectraPen LM500手持式光谱仪、PlantPen手持式光谱仪、FMT150藻类培养与在线监测系统、MC1000 8通道藻类培养与在线监测系统、MicroMac1000全自动营养盐分析监测系统、FMS CO2/O2呼吸测量分析系统等，并与中科院植物所、中科院水生所、中国农科院、陕西师范大学等建立了长期技术合作交流关系。欢迎联系开展实验合作与技术培训。邮箱：eco-lab@eco-tech.com.cn，info@eco-lab.cn; 电话：010-62615899

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