19. Filter Paper Medium (滤纸培养基)
(NH4)2SO4 1g KH2PO4 1g
MgSO4.7H2O 0.7g NaCl 0.5g
Distilled water (蒸馏水) 1000ml A strip of filter paper (滤纸条) 6ⅹ1cm (厘米)
20. Soybean Cake Meal Agar (黄豆饼粉琼脂)
10% infusion of soybean cake meal (10%黄豆饼粉浸汁) 1000ml
Glucose (葡萄糖) 10g NaCl 2.5g
21. Sawdust, Wheat-bran Medium (木屑、麸皮培养基)
Sawdust of broad leaf tree 75g Wheat bran 25g
Preparation:Mix these two ingredients well . Add appropriate water and blend
(the amount of water added may be becided by kneading with fingers ). Fill the
mixture into broad mouth of bottles . Stick a hole on the filling, put the
cotton pulg in bottle mouth .
壳斗科植物或阔叶树的木屑 75克 麸皮 25克
制法:加水适量充分混匀,水量以手捏时指间有水珠为宜,装瓶并压紧,在中心处扎一小洞,堵上棉塞,20磅灭菌1小时。
22. Pine Block or Pine Sowdust Medium (松木条或松木屑培养基)
1. Cut 1021 cm pine biocks and immerse them in 1-2% sucrose solution . Alow
the biocks fully absorbing suger solution .Fill the biocks into broad month of
bottle . Park the bottle with paper . Autoclave at 20 lbs for 1 hr .
(将10ⅹ2ⅹ1厘米的松木条浸泡在1-2%的蔗糖水中。使木条吸足糖水,装瓶不宜过紧,用纸包好,20磅灭菌1小时。)
2. Pine Sawdust Medium
Pine Sawdust (松木屑) 75% Rice bran (米糠) 23-24%
Socrose (蔗糖) 1-2% Water (appropriate)水 (适量)
Autoclave at 20 lbs for 1 hr.(20磅灭菌1小时)
23. Rice Straw. Dung Medium (稻草、马粪培养基)
Dried horse dung (or cow dung) (干马粪或牛粪) 65%
Died straw (干稻草) 35% Urea (尿素) 0.5%
Calcium superhosphate (过磷酸钙)0.7% Gypsum powder(石膏粉) 1%
Ammonium sulphate (硫酸铵) 0.3%
[Note]: Add appropriate water, pile them up and cause them fermentation.
(水适量进行堆积发酵)
24. Straw Rice Bran Medium (稻草、米糠培养基)
Dried straw (干稻草) 75-80% Rice bran (米糠) 20-25%
[Note]:Select no mouldy straw and cut them about 3 cm in length. Immerse them
in fresh water for 12 hr. Allow the straw fully (the amount of water added may
be decided by kneading with fingers. It is right when a drop of water appeared
between fingers). (
[注]:选无霉稻草,切成长约3厘米的小段,用清水浸泡12小时,使充分吸水,然后倾去水,加米糠和水,水量以用手捏时指间有水珠为宜。)
25. Rice Medium (米饭培养基)
Fill 10g of rice into a 50ml of Erlenmeyer flask. Add 20-30ml water. Autoclave
at 121℃ for 30 minutes (or fill 2g of rice into a 15ⅹ150mm tube). Add 6 ml of
water. Autoclave at 121℃ for 30 minutes.
(取大米10克,入50毫升椎形瓶中,水20-30毫升于15磅灭菌30分钟即成,或取大米2克,装入15ⅹ150毫米试管中,加水6毫升,于15磅灭菌30分钟。)
26. Filter Paper Medium (滤纸条培养基)
(NH4)2SO4 1g KH2PO4 1g
MgSO4.7H2O 0.5g Yeast Extract (酵母膏) 0.1g
Distilled Water (蒸馏水) 1000ml
Strip of filter paper 1ⅹ7 cm (滤纸条 1ⅹ7 厘米)
[Note]:Siddolve the above ingredients. Distribute the liqid medium into tubes
(each tube contains 1ml of the broth). Autoclave at 121℃ for 30 minutes. Then
put one or two sterilized strips of filter paper into a tube aseptically.
(将上述溶液分配成溶液后分装入试管,每管1毫升,15磅灭菌30分钟。再将灭菌的滤纸条(1条或2条)投入试管中即好使用。)
27. Yeast Extract-Medium Extract Agar I (酵母膏、麦芽膏琼脂)
Yeast extract (酵母膏) 10g Mall extract (麦芽膏) 10g
Glucose (葡萄糖) 4g Distilled water (蒸馏水) 1000ml
Agar (琼脂) 20g pH 7.0
适用范围:红霉素链霉菌