Protein Construct Expression and Purification Procedures (Gimila's Lab)
Protein Expression (Mark's Lab)
· Purification of GST Fused Proteins (Contributed by Chia Jin Ngee)
Many people have vented out frustration over insoluble GST-fused proteins. This is a protocol for enzymatically active soluble GST-fused proteins. All GST-fused proteins are rendered soluble with this technique though enzyme activitiy can range from 30-90%.
· Bacculovirus Production : for protein expression (Mike A. Dyer)
This is a modified version of the pharmingen Baculogold kit
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