免疫组织化学

·         Double Peroxidase (HRP) Immunohistochemical Labeling of Trypsin-Sensitive Antigens (KPL)

·         Immunohistochemistry (Tyner lab)
This is a basic immunohistochemistry protocol. Protocols for immunohistochemistry usr/localy widely, due to the differences between antigens and their recognition by antibody. For success it is crucial to try a number of different techniques and compare the results. 

·         Treptavidin/Beta-Galactosidase Immunohistochemical Labeling Procedure (KPL)  

• Immunohistochemical Staining of Floating Thick Sections (10-100 microns) (KPL)
  General Protocol for staining thick sections
    

• Immunohistochemical Staining for Formalin-Fixed, Paraffin-Embedded Tissues (LEP)
  This staining procedure is for tissues fixed in 10% neutral buffered formalin and embedded in paraffin
        

• Immunohistochemical Stain-Alkaline Phosphatase (APAAP) Technique (Nottingham Pathology Lab)
    

• Immunohistochemisty-Enzymatic Protocol (R&D)
  General protocol including details for sample preparation, tissue staining and trouble shooting
    

• Immunohistochemisty-Fluorescence Protocol (R&D)
  General protocol 
    

• Immunohistochemistry of Mouse Frozen Tissues (eBioscience) 
     

• Immunofluorescence- Whole Mount (Karl Clark)
  Immunohistochemistry allows visualization of antigens (usually proteins) within an embryo. Typically, a primary antibody binds specifically to an antigen (e.g. Luciferase); then a secondary antibody conjugated to a flourochrome or enzyme is bound to the heavy chain constant region of the primary antibody with specificity to the species of origin of the primary (e.g. anti-rabbit conjugated to Rhodamine
    

• Immunohistochemistry on Whole-Mount Embryos (Vesicle Trafficking)
    

• Wholemount Staining of Embryos (XMMR)
    

• Beta-gal Staining- Whole Mount (Karl Clark)
  Beta-gal staining allows identification of embryonic tissues/cells expressing lacZ marker protein by development of pigmented (blue) product in the presence of lacZ enzymatic activity.
   

• X-gal & AP Staining Protocol (Cepko Lab)
  Very detailed protocol with background information and several applications.
    

• Reducing Background Staining in Immunohistochemistry (KPL)
    

• Histochemical Visualization of Mycoplasma paratuberculosis (KPL)
     

• Solutions and Calculations for Immunohistochemistry (KPL)
    

• A semi-permenant mounting medium for immunofluorescence microscopy (House Ear Institute)
  A recipe for Moviol,
  an inexpensive mounting medium that is easy to prepare.
    

• Microwave Immunohistochemistry(Energy Beam Sciences, Inc.)
  Introduction to microwave immunohistochemistry technique and its applicaiton protocol 
    

• Antigen Retrieval : for immunohistochemistry (Mike A. Dyer)
    

• Dissociated Cell Staining : immunohistochemistry (Mike A. Dyer)