immunofluorescence of rabbit anti-murine VEGF by Peprotech

实验概要

The following protocol provides a method of immunofluorescence of rabbit anti-murine VEGF by Peprotech.

实验步骤

The following protocol used normal murine kidney tissue with an autostainer.

1. Deparaffinize and rehydrate the tissue section.

2. Perform heat-induced antigen retrieval with a high pH buffer for 20 minutes. Allow the slide to cool for 20 minutes.

3. Wash the slide for 5 minutes (TBS/Tween 20).

4. Use the following autostainer protocol:
    1) Incubate the tissue section for 1 hour at room temperature with Rabbit Anti-Murine VEGF at 2.5 μg/mL.
    2) Wash the slide.
    3) Incubate the tissue section with a fluorescent conjugated secondary antibody for 30 minutes at room temperature.
    4) Wash the slide.

5. Counterstain the tissue section with DAPI.