Lys(Dde)是用于制造分支多肽,构建侧链环肽和进行Lys侧链荧光修饰的常用工具.

它的zei大优点就是可以在保留多肽其它侧链保护基团的时候,单独被去除;也可以在去除其它保护基团的同时单独被保留.



Dde基团去除方法:用含2%水合肼的DMF(2% Hydrazine in DMF/DCM)反应30分钟


Method of selective removal of DDe with 2% hydrazine in DMF.


Resin

1. Place the peptidyl-Resin in a flask and treat with 2% hydrazine monhydrate in DMF (25ml/g). Stopper the flask and leave to stand at rt for 3 min.

2. Filter the resin and repeat the hydrazine treatment two more times. Wash the partially protected resin with DMF.

Peptide

1. Dissolve the Dde protected peptide with 2% hydrazine monhydrate in DCM(5mg/ml), stir at rt for 30 min.

2. Remove the DCM with vacuum.

3. Precipitate the deprotected peptide with cool ether and purified with HPLC.
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