nrStar™ Human tRF & tiRNA PCR Array包含了185类来自权威数据库收录和最新文献报道的tRF或tiRNA，方便客户对tRF & tiRNA进行简单快捷的表达谱分析。
    作为一种新型的tRNA来源的非编码小RNA，tRF& tiRNA由精确调控的生物发生过程所产生，参与行使多种生物学功能: 作为microRNA参与RNA干扰；调节靶向mRNA的稳定性；在细胞应激条件下促进应激颗粒(SG)的组装；调控细胞凋亡[1]；作为表观遗传因子在世代间传递[2,3](图1)。tRFs& tiRNA的组成和含量高度依赖于细胞类型，并且与多种病理状态密切相关。它们在生物体液中广泛存在，含量远超microRNA，使之有望成为极佳的分子标志物[4-7]。
    为了获得最佳的特异性和灵敏性，每一步qPCR流程都经过了精心优化和严格测试。每块板都设置了Spike-in对照、PCR阳性对照、基因组DNA对照和看家内参对照，确保了最高的数据质量。

产品列表芯片特点
· 前沿—近年来tRF & tiRNA研究呈井喷式的增长
· 专注—专注于出现频次高、生理病理关联性强的tRF&tiRNA类群
· 严谨—每一对qPCR引物都经过精心地优化和严格地测试
· 便捷—随取随用，极致便捷；标准qPCR板型直接测样，无需预扩增

工作流程
适用实时荧光定量PCR仪：
ABI ViiA™ 7, ABI 7500 & ABI 7500 FAST, ABI 7900HT, ABI QuantStudio™ 6 Flex Real-Time PCR system, ABI QuantStudio™ 7 Flex Real-Time PCR system, ABI QuantStudio™ 12K Flex Real-Time PCR System, Bio-Rad CFX384, Bio-Rad iCycler & iQ Real-Time PCR Systems, Eppendorf Realplex, QIAGEN Rotor Gene Q 100, Roche Light Cycler 480, Stratagene Mx3000, Roche Light Cycler 4

Human tRFs&tiRNAs (185) [7,8]

5001A, 5001B, 5002A, 5002C, 5003/4B, 5003/4C, 5005A, 5005B2, 3001A, 3002B, 3004A, 3004B, 3006B, 3031B, 3033A, 3030A, 3030B, 3016A, 1001, 1003, 1004, 1005, 1006, 1007, 1010, 1012, 1013, 1015, 1020, 1023, 1024, 1025, 1026, 1027, 1028, 1029, 1030, 1031, 1032, 1033, 1035, 1036, 1037, 1038, 1039, 1040, 1041, 1042, 5002B, 5005B, 5005C, 5007A, 5007C, 5008B, 5008C, 5009A, 5009B, 5009C, 5010A, 5011A, 5012B, 5013B, 5013C, 5015/17A, 5016A, 5016C, 5017B, 5017C, 5019A, 5019B, 5020/21A, 5020B, 5020C, 5021B, 5022A, 5022B, 5023A, 5023B, 5024A, 5026A, 5026/27B, 5026/27C, 5028/29A, 5028/29B, 5028C, 5030B, 5030C, 5032A, 5032B, 3002A, 3003A, 3003B, 3006A, 3008A, 3008B, 3009A, 3009B, 3010/11/12/13/14B, 3011/12A, 3013A, 3014A, 3015A, 3015B, 3016/18/22B, 3017A, 3018A, 3017B, 3019/20/21B, 3023B, 3024/25/29B, 3026B, 3027/28B, 3019A, 3020/21A, 3022A, 3026/27/28A, 3029A, 3031A, TRF21-26, TRF62, TRF63, TRF23, TRF204, TRF205, TRF208, TRF214/215, TRF223, TRF250, TRF272/274, TRF273, TRF293/294, TRF303/304, TRF305/306/307, TRF308, TRF312, TRF314, TRF315, TRF316, TRF318, TRF320, TRF321, TRF322, TRF323/324/326, TRF325, TRF327, TRF330, TRF337-339, TRF345, TRF346, TRF347, TRF351, TRF352, TRF353, TRF354, TRF356/359, TRF360, TRF368, TRF366, TRF365, TRF373, TRF374, TRF375, TRF393, TRF396, TRF398, TRF417, TRF419, TRF422, TRF457, TRF460, TRF462, TRF463, TRF466/468/469/471/472/473, TRF490, TRF492, TRF493, TRF511, TRF522/523/525, TRF524, TRF533/534, TRF537, TRF539, TRF546/547, TRF550/551, TRF552

参考文献
1. Anderson, P. & Ivanov, P. tRNA fragments in human health and disease. FEBS letters 588, 4297-4304, doi:10.1016/j.febslet.2014.09.001(2014). [PMID: 25220675].
2. Chen, Q. et al. Sperm tsRNAs contribute to intergenerational inheritance of an acquired metabolic disorder. Science 351, 397-400, doi:10.1126/science.aad7977(2016). [PMID: 26721680].
3. Sharma, U. et al. Biogenesis and function of tRNA fragments during sperm maturation and fertilization in mammals. Science 351, 391-396, doi:10.1126/science.aad6780(2016). [PMID: 26721685].
4. Dhahbi, J. M. et al. 5' tRNA halves are present as abundant complexes in serum, concentrated in blood cells, and modulated by aging and calorie restriction. BMC genomics 14, 298, doi:10.1186/1471-2164-14-298(2013). [PMID: 23638709].
5. Schageman, J. et al. The complete exosome workflow solution: from isolation to characterization of RNA cargo. BioMed research international 2013, 253957, doi:10.1155/2013/253957(2013). [PMID: 24205503].
6. Telonis, A. G. et al. Dissecting tRNA-derived fragment complexities using personalized transcriptomes reveals novel fragment classes and unexpected dependencies. Oncotarget 6, 24797-24822, doi:10.18632/oncotarget.4695(2015). [PMID: 26325506].
7. Olvedy, M. et al. A comprehensive repertoire of tRNA-derived fragments in prostate cancer. Oncotarget, doi:10.18632/oncotarget.8293(2016). [PMID: 27015120].
8. Kumar, P., Mudunuri, S. B., Anaya, J. & Dutta, A. tRFdb: a database for transfer RNA fragments. Nucleic acids research 43, D141-145, doi:10.1093/nar/gku1138(2015). [PMID: 25392422].