NFkB Signaling Pathway EpiTect ChIP qPCR Array 

NFκB信号通路ChIP qPCR芯片
 

Product	Species	Technology	Cat. No.
NFkB Signaling Pathway EpiTect ChIP qPCR Array	Human	Histone Modifications	GH-025A
NFkB Signaling Pathway EpiTect ChIP qPCR Array	Mouse	Histone Modifications	GM-025A

The Human NFκB Signaling Pathway EpiTect ChIP qPCR Array profiles the histone modification status or “histone code” of 84 genes related to NFκB-mediated signal transduction. Histone modifications regulate chromatin structure and correlate with the transcriptional activity of associated genes. The NFκB family is a group of transcription factors activated by stress and inflammatory responses. Other NFκB stimuli include growth factors, cytokines, pathogen-associated molecular products (PAMPs), and DNA damage. NFκB-responsive genes regulate processes such as apoptosis, tissue remodeling, tumorigenesis, and inflammation. The NFκB transcriptional response also involves epigenetic factors such as histone deacetylases (HDACs) to enhance NF?B action on target gene promoters. Analyzing histone modifications of the NFκB pathway and NFκB target genes simultaneously can provide insights into the molecular and epigenetic mechanisms regulating NFκB signaling. This array includes members of the Rel, NFκB, and IκB families, NFκB targets, and the extracellular ligands and cell surface receptors that activate this pathway. Using chromatin immunoprecipitation and this real-time PCR array, research studies can easily and reliably analyze the histone modification patterns associated with a focused gene panel involved in NFκB signal transduction.
NFκB信号通路ChIP qPCR芯片分析NFκB介导的信号通路相关的48个关键基因的组蛋白修饰状态或组蛋白密码。组蛋白修饰调节染色质结构和与相关基因的转录活性。NFκB家族是一组转录因子由压力和炎症反应激活。其他NfκB可以刺激生长因子、细胞因子,病原体相关分子产物(PAMPs)和DNA损伤等。NFκB应答基因调控过程,如细胞凋亡、组织重构、肿瘤发生和炎症。NFκB转录反应还涉及表观遗传因子,如组蛋白去乙酰酶抑制剂(hdac)，增强NFκB在靶标基因启动子上的活性。NFκB通路分析组蛋白修饰和NFκB靶标标基因，可以助于洞察调节NFκB信号通路的分子和表观遗传机制。NFκB,这个芯片包含Rel, NFκB和 IκB的家族成员, NFκB靶标基因，以及激活这个通路的细胞外配体和细胞表面受体。
Activation of NFκB Pathway:
Ligands and Receptors: IL1B, IL8, MYD88, TLR1, TLR2, TLR3, TLR4, TLR6, TLR8, TOLLIP, TNF, TNFRSF10A, TNFRSF10B.
Kinases: CHUK (IKKa), IRAK1, IRAK2.
IκB kinase/NFκB Cascade: EDARADD, IKBKB, IRAK2, STAT1, TRAF2, TLR8.
Cytoplasmic Sequestering and Release of NFκB: BCL3, IL10, NLRP12 (NALP12), NFKBIA (IkBa/MAD3), CD27 (TNFRSF7), TNFSF14.
Transcription Factors: NFKB1, NFKB2, NFKBIA, RELA, RELB, STAT1, TNF.
Inflammatory Response: IL1B, IL8, IRAK2, MYD88, NFKB1, TLR1, TLR2, TLR3, TLR4, TLR6, TLR8, TNF.
Positive Regulation of IκB Kinase and the NFκB Cascade:
Ligands and Receptors: CD40 (TNFRSF5), F2R, FASLG (TNFSF6), LTBR, SLC20A1, TICAM2 (TRAM), TNFRSF1A, TNFSF10 (TRAIL), TICAM1 (TRIF).
Membrane Molecules: GJA1, HMOX1, LPAR1 (EDG2), RHOA.
Kinases: RIPK1, TBK1.
Other: BCL10, BIRC2 (c-IAP2), NOD1 (CARD4), CASP1 (ICE), CASP8 (FLICE), CFLAR (CASPER), FADD, IKBKE, MALT1, PPM1A, REL, TRIM13 (RFP2), TRADD.
NFκB Responsive Genes:
Extracellular and Adhesion Molecules: AGT, CSF1 (MCSF), CSF2 (GM-CSF), CSF3 (GCSF), ICAM1, IFNA1, IFNG.
Cytokines: CCL2 (MCP-1), CCL5 (RANTES), CSF1 (MCSF), CSF2 (GM-CSF), CSF3 (GCSF), IFNA1, IFNG, IL8, IRF1, LTA (TNFB), TNF.
Apoptosis: BCL2A1 (Bfl-1/A1), BCL2L1 (BCL-X), BIRC3 (c-IAP1).
Other Factors Involved in the NFκB Pathway: 
Ligands and Receptors: IL1R1, RAF1, TLR7, TLR9.
Kinases: AKT1, RAF1.
Transcription Factors: ATF1, EGR1, ELK1, FOS, JUN, TNFAIP3
Inflammatory Response: CCL2 (MCP-1), FOS, IL1A, IL1R1, TLR7, TLR9.