uProt™人类蛋白质芯片最早由美国约翰霍普金斯大学开发,它覆盖了16368种人类蛋白质,后经过不断升级,目前含有19394个人重组蛋白,是迄今为止最高通量人重组蛋白质组芯片。每个蛋白质都是由真核表达系统表达及纯化的,并且设置有重复点。
HuProt™人类蛋白质组该芯片从人类蛋白质组学角度全局揭示人类疾病尤其是肿瘤性疾病的发生、发展以及用药以后的作用机理。该芯片已经广泛应用在许多科研领域。例如:血清图谱构建、抗体特异性鉴定、蛋白质相互作用、泛素和SUMO的E3连接酶和蛋白质激酶等底物筛选研究。
蛋白芯片应用产品性能 人蛋白质:19,394种
一张芯片上的点阵数目:1
点样环境:温度4-8℃,湿度30%~40%
芯片基片:FAST,尺寸为75*25 mm
点直径:~60微米
样品重复:每个样品重复两个点
点体积:0.5~1 nL/点
芯片点制后处理:4℃过夜固定后-80℃长期保存
发表的文章1. Fan, Q
et al. (2014). Identification of proteins that interact with alpha A-crystallin using a human proteome microarray.
Mol Vis 20:117–124.
[PubMed]2. Deng, RP
et al. (2014). Global Identification of O-GlcNAc Transferase (OGT) Interactors by a Human Proteome Microarray and the Construction of an OGT Interactome.
Proteomics 14(9): 1020-30. doi: 10.1002/pmic.201300144. Epub 2014 Mar 25.0.
[PubMed]3. Ma, TM
et al. (2014) Serine Racemase Regulated by Binding to Stargazin and PSD-95: Potential NMDA-AMPA Glutamate Neurotransmission Cross-talk. J Biol Chem pii: jbc.M114.571604. [Epub ahead of print]; First Published on August 27, 2014.
[PubMed]4. Barry G
et al. (2013). The long non-coding RNA Gomafu is acutely regulated in response to neuronal activation and involved in schizophrenia-associated alternative splicing.
Molec Psychiatry Apr 30. doi: 10.1038/mp.2013.45. ePub ahead of print.
[PubMed]5. Lee YI
et al. (2013). Protein microarray characterization of the S-nitrosoproteome.
Mol Cell Proteomics 13:63-72.
[PubMed]6. Chen Y
et al. (2013). Bcl2-associated Athanogene 3 Interactome Analysis Reveals a New Role in Modulating Proteasome Activity.
Mol Cell Proteomics 12(10):2804-19.
[PubMed]7. Donnelly CJ
et al. (2013). RNA toxicity from the ALS/FTD C9ORF72 expansion is mitigated by antisense intervention.
Neuron 80(2):415-28.
[PubMed]8. Fan B
et al. (2013). A human proteome microarray identifies that the heterogeneous nuclear ribonucleoprotein K (hnRNP K) recognizes the 5’ terminal sequence of the hepatitis C virus RNA.
Mol Cell Proteomics. 13(1):84-92.
[PubMed]9. Tarrant MK
et al. (2012). Regulation of CK2 by phosphorylation and O-GlcNAcylation revealed by semisynthesis.
Nat Chem Biol 8(3):262-9.
[PubMed]10. Jeong JS
et al. (2012). Rapid identification of monospecific monoclonal antibodies using a human proteome microarray.
Mol Cell Proteomics 11(6):O111.016253.
[PubMed]11. Huang Y.
et al. (2012). Global tumor protein p53/p63 interactome: making a case for cisplatin chemoresistance.
Cell Cycle 11(12):2367-79.
[PubMed]12. Hu CJ
et al. (2012). Identification of new autoantigens for primary biliary cirrhosis using human proteome microarrays.
Mol Cell Proteomics 11(9):669-80.
[PubMed]