Abstract：

　　In this study we evaluated the performance of a Preparative SFC‐MS system combining the JASCO Preparative SFC with the Advion expression CMS (Compact Mass Spectrometer) utilizing a simplified passive splitter for mass‐directed fraction collection. The initial test explored a wide range of flow rates both isocratic and gradient to test performance and reliability of the passive splitter, coinciding timing of MS and UV signal and the clearance of the source with highly concentrated samples. The system was further evaluated testing the recovery of main peaks as well as small low level impurities using either TIC or XIC fraction triggering. Purity of the collected peaks was also determined.