qTOWER384荧光定量基因扩增仪参数指标

参数指标

Technical Data

Thermal block
Sample block	Aluminum, special alloy
Block capacity	384 wells microplates
Sample volume	2–30 μl (5–20 μl recommended)
Heating	Max. 4 °C/s
Cooling	Max. 2 °C/s
Adjustable temperature range	4 °C to 99 °C
Temperature uniformity	55 °C ± 0,15 °C after 15 sec
Temperature control accuracy	± 0,1 °C
Gradient	Linear Gradient Tool
Max./Min. Gradient	24 °C/0.1 °C
Heated lid
Lid temperature	30 °C to 110 °C
Contact pressure	30 kg, automated
Optics
Measuring principle	Fibre optic shuttle system with 16fold scanner and color modules for excitation and emission filters
Light source	4 long-life, high-power LEDs (RGBW)
Detector	High sensitive CPM (Channel Photo Multiplier)
Color modules	12 Color-, FRET- and Protein modules 6 positions inside device
Read-out time	6 sec for 384 wells independent of the number of dyes
Control
Control	PC software
Time inc/dec	± 1 to 240 sec/cycle
Temperature inc/dec	± 0,1 to 20 °C/cycle
Dimensions
Weight	Approx. 30 kg
Dimemsions (W x H x L)	275 mm x 585 mm x 275 mm
Additional technical data
Interfaces	PC connection: USB
Noise emission	Max. 45 db
Warranty	2 years warranty on device system 10 years long-term warranty on high performance optics

Parameters: color modules
Name	Excitation	Emission	Dyes* (examples)
Color module 1	470 nm	520 nm	FAMTM, Sybr®Green, Alexa488®
Color module 2	515 nm	545 nm	JOETM, HEXTM, VIC®, YakimaYellow®
Color module 3	535 nm	580 nm	TAMRATM, DFOTM, Alexa546®, NEDTM
Color module 4	565 nm	605 nm	ROXTM, TexasRed®, Cy3.5®
Color module 5	630 nm	670 nm	Cy5®, Alexa633®, Quasar670TM
Color module 6	660 nm	705 nm	Cy5.5®, LightCycler Red®
FRET module 1	470 nm	580 nm	FAMTM (donor)/TAMRATM(acceptor)
FRET module 2	470 nm	670 nm	FAMTM (donor)/Cy5®(acceptor)
FRET module 3	470 nm	705 nm	FAMTM (donor)/Cy5.5®(acceptor)
FRET module 4	515 nm	670 nm	JOETM (donor)/Cy5®(acceptor)
FRET module 5	470 nm	605 nm	FAMTM (donor)/ROXTM(acceptor)
Color modul Protein 1	490 nm	580 nm	SYPRO® Orange

* Yakima Yellow is registered trademark of Epoch Biosciences, Inc. Cy is a trademark of GE Healthcare. FAM, HEX, JOE, VIC, TAMRA, NED and ROX are trademarks of Applera Corporation or its subsidiaries in the US and/or certain other countries. SYBR, Alexa Fluor, SYPRO and Texas Red are registered trademarks of Molecular Probes, Inc. TaqMan and LightCycler are registered trademarks of Roche Group, Inc. Quasar Dyes are trademarks of Biosearch Technologies Inc. DFO™ is a trademark of Eurogentec S.A. Windows and Excel are trademarks of Microsoft Corporation.

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