SOLiD 5500测序系统(...

SOLiD 5500测序系统(二代测序)参数指标

参数指标我要纠错

5500 W Series Genetic Analyzer V1.0 Specifications

System and Features5500 W System
(1 FlowChip)
5500xl W System 
(2 FlowChips)  

按每条泳道收费测序 Reagent consumption engineered independently for each lane; users pay only for the consumables in the active lanes when performing a partial run.
Application-Per-Lane Sequencing Independent FlowChip lanes allow users to configure read length of chemistry for each lane enabling multiple applications in a single run.
多重 96 barcodes for both RNA and DNA applications.
Instrument Throughput1  

1 x 50
1 x 75
2 x 50 MP
  

~ 80 Gb
~ 120 Gb 
~ 160 Gb
  

~ 160 Gb
~ 240 Gb
~ 320 Gb

Exomes/run2 高达 18% 高达 36%
Transcriptomes/run3 高达 12% 高达 24%
Small RNA/run4 高达 144% 高达 288%
Maximum Read Lengths5 1 x 75 Frag, 2 x 50 MP

 

  1. Instrument throughput is estimated using typical runs at a density of 700,000/panel, 65%-70% passing filter. Actual throughput will depend on the samples being run. Throughput uses the quality reads from the instrument that pass the primary analysis filter.

  2. ~50 Mb exome, >100x average coverage using fragment chemistry, 1 x 75 bp run.

  3. Transcriptome assumes > 100 million reads/sample using fragment chemistry, 1x 50 bp run.

  4. ~8 million reads/sample for each small-RNA sample and utilizing barcodes (48).

  5. New Wildfire paired-end (wPE) 50 bp x 50 bp early access to start by the end of 2012.


 

技术参数

项目

5500xl SOLiD System

5500 SOLiD System

适用范围快速、性价比高的大型基因组研究高效经济的小规模研究
系统准确性高达99.99%Up to 99.99%
可定位的数据通量/天高达20-30 GbUp to 10-15 Gb
样品/运行 2 个基因组
 24 个外显子组
 12 个转录组
 1 Genome
 12 Exomes
 6 Transcriptomes
独立运行通道1-121-6
读长 Fragment: 75 bp
 Paired-end: 75 bp x 35 bp
 Mate-pair: 60 bp x 60 bp
 Fragment: 75 bp
 Paired-end: 75 bp x 35 bp
 Mate-pair: 60 bp x 60 bp
运行时间35 bp,1天(1个通道)
75 bp x 35 bp,7天(12个通道)
60 bp x 60 bp,7天(12个通道)
35 bp,1天(1个通道)
75 bp x 35 bp,7天(12个通道)
60 bp x 60 bp,7天(12个通道)
多重分析条形码96个,适合RNA和DNA96个,适合RNA和DNA
标准可定位数据90 Gb180 Gb
说明书

5500xl SOLiD System(PDF)

5500 SOLiD System(PDF)

 




相关基因/DNA测序仪