Orbitrap Fusion Tribrid轨道离子阱质谱仪参数指标

扫描速率QTMS²：15Hz

扫描速率ITMS²：20Hz

分辨率：450,000

质量精度：3ppm ext，1ppm int

裂解模式：CID、HCD、ETD

分析器：Q 、 QTMS、ITMS

检测器：ion Trap，Orbitrap

离子源：赛默飞EASY-NG™离子源

离子光学：S-透镜

尺寸：1186X674X650mm

线性离子阱质量范围：

标准 – m/z 50–2,000

高质量范围 – m/z 200–4,000

Orbitrap质量范围

没有母离子选择时，zei高m/z 6000

分辨率：

15,000–450,000 (FWHM) at m/z 200

采集速度

Orbitrap MSn up to 15 Hz

Ion trap MSn up to 20 Hz

质量准确度：

<3 ppm RMS 外标

<1 ppm RMS 内标

MS/MS Electrospray Ionization (ESI) Ion Trap 灵敏度

2 μL of a 50 fg/μL solution of reserpine (100 fg total) injected at a flow of 500 μL/min will produce a minimum signal-to-noise ratio of 100:1 for the transition of the isolated protonated molecular ions at m/z 609 to the largest two product ions, m/z 397 and m/z 448, when the mass spectrometer is operated at unit resolution in the full-scan MS/MS mode, m/z 165–615. The test requires the HESI probe.

100 fg 利血平，单位质量分辨下，全扫描MS/MS， S/N 100:1

动态范围：

>5,000单张谱图内 within a single scan, guaranteeing specified mass accuracy

MS Scan Power 采集级数

MSn, for n = 1 through 10

同步母离子选择Synchronous Precursor Selection

Up to 15 precursors per MS2 scan, for MS3 analysis only

Multiplexing Using Ion-Routing Multipole

Up to 10 precursors per scan using quadrupole mass filter

极性转换Polarity Switching

One full cycle in 1.1 sec (one full scan in positive mode and one full scan in negative mode at resolution setting of 35,000)

1张全谱1.1秒（正负切换采集全谱，分辨率为35,000）

ETD选项

3 μL/min流速下进样1 pmol/μL angiotensin I 溶液，ETD碎裂效率 >15%