Sony SP6800流式光谱...

Sony SP6800流式光谱分析仪 参数指标

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                光学系统                   激光参数
                波长                488 nm Blue (40mW) ，635nm Red (60 mW)
                光学探测系统
                滤光器                无
                光电倍增管（PMT）                1个，32 通道
                通道数                2条散射光, 14色荧光（包括PI等），自发荧光以及时间
                检测信号范围                500 nm – 800 nm
                检测系统                  液流速率                18 – 60 μL/min
                死体积                50nL
                样品zei小检测体积                100 μL
                分析速率                20,000 eps (max)
                灵敏度(MESF)                FITC = 300 (typical failure~120)， PE = 200 (typical ~ 80)
                滞留率< 0.1%
                样品管                12 x 75 mm, 5mL tubes (聚丙乙烯, 聚丙烯等材质)
                上样                单管上样
                射流室                10 L 鞘液槽 (已密封加压), 10 L 鞘液槽, 5 L 去离子水槽
                主机/电脑                     尺寸                63.0 cm x 60.0 cm x 72.0 cm
                电源                100   V 50/60 Hz, 120 V 60 Hz
                耗电量                500   W（zei大）
                操作温度                17.5   to 27.5°C
                湿度                20%   to 80% (不会造成血液凝固)
                数据格式                流式细胞分析术的标准(FCS) 3.0或3.1
                电脑                VAIO   L系列一体式电脑，预装Windows&reg;8操作系统，专业版，64-bit




SP6800 instrument specification
InstrumentConfiguration2 laser model, 488/6382 laser model, 405/4883 laser model, 405/488/638
Model NumberLE-SP6800ZBLE-SP6800ZCLE-SP6800ZE
OpticsLaser Specification
LasersSemiconductor 2 laser modelSemiconductor 2 laser modelSemiconductor 3 laser model
488nm, 638nm405/488405/488/638
Maximum power (at flow cell)488nm 40mW405nm 60mW405nm 60mW, 488nm 40mW
638nm 60mW488nm 40mW638nm 60mW
Detection Optics
Scatter signalsForward scatter (FSC), Side scatter (SSC)
Spectroscopic methodPrism array spectroscopy
Fluorescent channels32 channel PMT (wavelength: 500-800nm)32 channel PMT (wavelength: 500-800nm) PMT x 2(420-440nm, 450-470nm)
SystemSignal ResolutionHeight 20 bit, Area 32 bit Sampling frequency: 50MHz
Measurement parameters64 channels, FSC, SSC,66 channels, FSC, SSC, cell position XY
cell position XY
Pulse shape parametersHeight, Area, Width
Optical alignmentAutomated alignment with Corefinder™ technology
Event Rate10,000eps (standard)/20,000eps (maximum)
FluidicsSample loader typeSingle auto-loader
Supporting sample tube5ml polystyrene round tubes
Sheath Flow SpeedLow (approx. 3m/s), Mid (approx. 5m/s), High (approx. 10m/s)
Detection channel dimensions200μm x 200μm
PerformanceFluorescence sensitivityFITC: 120 MESF / PE: 70 MESF
LinearityFITC R2 ≧0.995 / PE R2 ≧0.995
Detection size range0.5~40μm (beads)
Fluorescence detection resolution488nm Laser/CH16, 638nm Laser/CH27* : ≧2.5% (HPCV)
SoftwareUnmixing algorithmsSpectrum method (LSM, WLSM, PSA and Constraint option), Reverse matrix method (Conventional)
AutofluorescenceAutofluorescence spectral detection
Virtual FilterPossible to change wavelength region for each fluorochromes in analysis
Export Data FormatFlow Cytometry Standard (FCS) 3.0, 3.1
Main UnitDimensionsMain unit: Width 60.0cm x Depth 63.5cm x Height 71.3cm
Fluidics cart: Width 78.6cm x Depth 52.1cm x Height 58.0cm
　
WeightMain unit: approx. 99kg (dried weight)/Fluidics cart: approx. 32kg (dried weight)
Air pressure supply350kPa~450kPa (51psi~65psi)
AC Power supplyAC100V 50/60Hz, AC120V 60Hz
Power consumption220W (max)
Operating temperature16-29 degrees Celsius, Room temperature variation: within 5 degrees Celsius
Operating humidity20% to 80% (condensation free)
Recommended PCSP6800 workstation

*Except for LE-SP6800ZC (This HPCV value is calculated for red laser)

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