产地：MBI 货号：ER1371 Conditions for 100% Activity 1X Buffer Tango™: 33 mM Tris-acetate (pH 7.9 at 37°C), 10 mM Mg-acetate, 66 mM K-acetate and 0.1 mg/ml BSA. Incubate at 37°C. Storage Buffer SchI is supplied in: 10 mM Tris-HCl (pH 7.5 at 25°C), 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol. Ligation and Recleavage After 10-fold overdigestion with SchI, approximately 90% of DNA fragments can be ligated and more than 90% of these can be recut.
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