产地：MBI 货号：ER1381 Conditions for 100% Activity 1X Buffer Tango™: 33 mM Tris-acetate (pH 7.9 at 37°C), 10 mM Mg-acetate, 66 mM K-acetate and 0.1 mg/ml BSA. Incubate at 37°C. Storage Buffer XapI is supplied in: 10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol. Digestion of Agarose-embedded DNA Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours. Ligation and Recleavage After 10-fold overdigestion with XapI, more than 95% of the DNA fragments can be ligated and recut.
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