Cloning of restriction enzyme generated DNA fragments.
Cloning of PCR products.
Joining of double-stranded oligonucleotide linkers or adaptors to DNA.
Site-directed mutagenesis.
Amplified fragment length polymorphism (AFLP).
Ligase-mediated RNA detection (3).
Nick repair in duplex DNA, RNA or DNA/RNA hybrids.
Self-circularization of linear DNA.
Description
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids, joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids (1, 2).
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